Part:BBa_K4181016

Tyrosinase protein DNA sequence

And that sequence is used to produce tyrosinase which reacts with levodopa to produce melanin, which is used to protect the DNA.

Usage and Biology

The coding sequence of tyrosinase was present in the genome of Bacillus polymorpha, but we were unsuccessful in borrowing the Bacillus polymorpha strain, so we still chose to synthesize tyrosinase directly by subscription. The sequence was synthesized by removing the start codon and adding the linker sequence at the 5' end and adding a 6*His tag before the stop codon at the 3' end for subsequent detection.

Figure 1. Image of tyrosinase-encoding gene by PCR followed by electrophoresis. The picture indicates a positive result.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 298
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 298
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 303
  Illegal XhoI site found at 491
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 298
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 298
  
• 1000
  COMPATIBLE WITH RFC[1000]