Regulatory

Part:BBa_K4244006

Designed by: Arthur Vancolen   Group: iGEM22_Wageningen_UR   (2022-10-08)
Revision as of 10:21, 12 October 2022 by Sarah99 (Talk | contribs)

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OmpC

OmpC is a porin gene, whose promoter is controlled by the transcription factor OmpR, part of the EnvZ/OmpR two component system. The EnvZ/OmpR two-component system in normal conditions controls the expression of the ompC and ompF porin genes, controlling iron homeostasis (1). This part is native to </i>Escherichia coli</i>, which means that it has directly been amplified from the organism that we are working on: </i>E. coli</i> Nissle 1917. Since this part was originally upstream the OmpC gene, it also contains its native rbs. E. coli OmpC without rbs is under the iGEM registry code BBa_K769001.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Gerken H, Vuong P, Soparkar K, Misra R. Roles of the EnvZ/OmpR Two-Component System and Porins in Iron Acquisition in Escherichia coli. mBio. 2020;11(3):e01192-20. Published 2020 Jun 23. doi:10.1128/mBio.01192-20

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