![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4201017
Designed by: David Birkhaeuser Group: iGEM22_CU-Boulder (2022-10-12)
CrtE_cytoTDS2_RUBY
This construct is split up into three main gene segments. At the center of each segment lies a gene of interest, in this case CrtE, cytoTDS2, and RUBY, respectively. Each segment is flanked upstream by the promoter Gmubi and downstream by the terminator AtHSP. CrtE encodes for an enzyme that synthesizes GGPP1. The GGPP is then used to make taxadiene by taxadiene synthase, encoded by cytoTDS22.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2087
Illegal PstI site found at 5638
Illegal PstI site found at 10846 - 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI site found at 948
Illegal BsaI site found at 1887
Illegal BsaI site found at 2167
Illegal BsaI site found at 3113
Illegal BsaI site found at 5438
Illegal BsaI site found at 5718
Illegal BsaI.rc site found at 934
Illegal BsaI.rc site found at 1873
Illegal BsaI.rc site found at 2153
Illegal BsaI.rc site found at 3100
Illegal BsaI.rc site found at 5424
Illegal BsaI.rc site found at 5704
Illegal BsaI.rc site found at 6651
[edit]
Categories
Parameters
None |