Coding
Part:BBa_K4488005
Designed by: Sylvie Nolf Group: iGEM22_Sydney_Australia (2022-09-30)
Anti-GFP Nanobody/sdAb
Nanobody binding sfGFP. Optimised for expression in Escherichia coli. Binding to a fluorescent protein allows for fluorescent tagging and testing of constructs. PDB: 3OGO-H. Esp3I golden gate sites flank either end of this part. Analogous to BBa_K929102
Usage: This part has been used in a composite part with Neae-Intimin for expression in TOP10 E.coli. See part BBa_K4488027.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |