Composite

Part:BBa_K4121052

Designed by: Ziyu Li   Group: iGEM22_NEFU_China   (2022-10-05)
Revision as of 09:42, 12 October 2022 by L7 (Talk | contribs)

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Expression cassette of TmCrtE with constitutive promoter

The composite part is called "pFBA1-TmCrtE-tTDH1". We use pFBA1 as the promoter, tTDH1 as the terminator and the CDS of this part is TmCrtE(from Taxus media). The three basic parts above make up a new transcription unit, it has the fuction to catalyze the conversion of farnesyl pyrophosphate (FPP) to heterologous geranylgeranyl diphosphate(GGPP).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 728
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 590
    Illegal BglII site found at 731
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 728
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 728
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None