Device
Part:BBa_K176220:Design
Designed by: Danqian Liu, Zongxiao He, Hao Jiang Group: iGEM09_USTC (2009-10-08)
cell density->GFP: pCon 0.04->luxI-LVA+pCon 0.70->luxR+pLux/Tet->GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 856
Illegal NheI site found at 879 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 698
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1834
Illegal BsaI.rc site found at 2578
Design Notes
Input: AHL
Output1: AHL
Output2: GFP
Output3: Cell Density
Design:
pCon 0.04(J23109) generates LuxI-LVA, LuxI-LVA generates Output1 AHL, the concentration of AHL in the medium depends on both LuxI-LVA level per cell and cell density.
pCon 0.70(J23101) generates LuxR.
pLux/Tet(K176000) generates Output2 GFP, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input AHL), repressed by TetR and no aTc. High level GFP inhibits cell growth to control Output3 Cell Density.
Source
K176086 I13504