Composite

Part:BBa_K4236102

Designed by: Hanpeng Chen   Group: iGEM22_iBowu-China   (2022-10-12)
Revision as of 09:09, 12 October 2022 by Haber (Talk | contribs)


T7-RBS-atF3H-atFLS+atUGT

The whole pathway in plants to produce astragalin contains three enzymes, F3H, FLS, and UGT. We want to construct a big plasmid consisting of three genes we have introduced to produce astragalin. In order to better express each enzyme, we tried to connect the smaller F3H and FLS with a linker to form a fusion protein, and the larger UGT was expressed separately. Therefore, AtF3H-AtFLS + AtUGT expression vector was constructed.

Usage and Biology

Other Information

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None