Composite

Part:BBa_K4172001

Designed by: liu jinrong   Group: iGEM22_XHD-Wuhan-China   (2022-10-12)
Revision as of 08:13, 12 October 2022 by Registry (Talk | contribs)

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CusS/CusR two component system+ribB

The part uses the pCusC promoter which is sensitive to copper via the E. coli CusS/CusR two component system. The promoter responds directly to the CusR cytoplasmic response regulator which is activated by a transmembrane histidine kinase enzyme CusS found in the cell membrane. A standard ribosome binding site was added to the composite device after the promoter.The composite ends with a double terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1373
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1263
    Illegal AgeI site found at 1305
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 767


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