Coding

Part:BBa_K283048:Design

Designed by: Shi Lei, W Ling , Nelson Chu   Group: iGEM09_HKU-HKBU   (2009-10-13)
Revision as of 08:20, 13 October 2009 by Registry (Talk | contribs)

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sp-eGFP-streptavidin-AIDAc


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 181
    Illegal EcoRI site found at 1675
    Illegal EcoRI site found at 2371
    Illegal PstI site found at 1723
    Illegal PstI site found at 2309
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 181
    Illegal EcoRI site found at 1675
    Illegal EcoRI site found at 2371
    Illegal PstI site found at 1723
    Illegal PstI site found at 2309
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 181
    Illegal EcoRI site found at 1675
    Illegal EcoRI site found at 2371
    Illegal BamHI site found at 17
    Illegal BamHI site found at 949
    Illegal BamHI site found at 2573
    Illegal XhoI site found at 2779
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 181
    Illegal EcoRI site found at 1675
    Illegal EcoRI site found at 2371
    Illegal PstI site found at 1723
    Illegal PstI site found at 2309
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 181
    Illegal EcoRI site found at 1675
    Illegal EcoRI site found at 2371
    Illegal PstI site found at 1723
    Illegal PstI site found at 2309
    Illegal AgeI site found at 991
    Illegal AgeI site found at 1042
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_K283007


Source

The CI, terminator, pLambda promoter and the chez coding region are self designed The rbs, and the pLux promoter are from the registry

References