Composite

Part:BBa_K4201016:Design

Designed by: David Birkhaeuser   Group: iGEM22_CU-Boulder   (2022-10-12)
Revision as of 07:41, 12 October 2022 by Dabirk (Talk | contribs)


Crte-cytoTDS-MBP_RUBY


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1002
    Illegal PstI site found at 4800
    Illegal PstI site found at 5648
    Illegal PstI site found at 10856
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1002
    Illegal PstI site found at 4800
    Illegal PstI site found at 5648
    Illegal PstI site found at 10856
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 790
    Illegal BglII site found at 1618
    Illegal BglII site found at 3156
    Illegal BglII site found at 4203
    Illegal BglII site found at 5582
    Illegal BglII site found at 6517
    Illegal BglII site found at 10419
    Illegal BamHI site found at 5254
    Illegal BamHI site found at 9068
    Illegal XhoI site found at 2579
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1002
    Illegal PstI site found at 4800
    Illegal PstI site found at 5648
    Illegal PstI site found at 10856
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1002
    Illegal PstI site found at 4800
    Illegal PstI site found at 5648
    Illegal PstI site found at 10856
    Illegal NgoMIV site found at 7004
    Illegal NgoMIV site found at 7583
    Illegal NgoMIV site found at 9296
    Illegal AgeI site found at 2321
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 948
    Illegal BsaI site found at 5448
    Illegal BsaI site found at 5728
    Illegal BsaI site found at 6675
    Illegal BsaI site found at 10656
    Illegal BsaI.rc site found at 934
    Illegal BsaI.rc site found at 5434
    Illegal BsaI.rc site found at 5714
    Illegal BsaI.rc site found at 6661
    Illegal BsaI.rc site found at 10642
    Illegal BsaI.rc site found at 10922
    Illegal SapI.rc site found at 3377
    Illegal SapI.rc site found at 3583


Design Notes

Part information and design consideration can be found on respective parts pages.


Source

Crte-cytoTDS-MBP is a de novo synthesized construct made for this project. Learn more about our construct and its genes by searching for BBa_K4201004.

RUBY is a reporter gene from the order Caryophyllales.

Gmubi promoters originate from Glycine max.

AtHSP terminators originate from the Arabidopsis thaliana genome.


Synthesis was completed by iGem sponsors IDT and Twist Biosciences.