Composite

Part:BBa_K4236100

Designed by: Haber Chen   Group: iGEM22_iBowu-China   (2022-10-12)
Revision as of 07:29, 12 October 2022 by Registry (Talk | contribs)

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T7-RBS-atF3H+atFLS

The whole pathway in plants to produce astragalin contains three enzymes, F3H, FLS and UGT. F3H is an enzyme of 41.36 kD, which catalyzes naringenin into dihydrokaempferol. FLS is of 39.41 kD to catalyze dihydrokaempferol into kaempferol. We constructed an intact plasmid to express AtF3H and AtFLS respectively.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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