Composite

Part:BBa_K4227021

Designed by: Yang Chen   Group: iGEM22_ZJUT-China   (2022-10-02)
Revision as of 07:22, 12 October 2022 by Kaga (Talk | contribs)

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Pxyl-TetR-ssrA

Pxyl is a kind of promotor that can be started by the xylose.

tetR-ssrA is an improved version of tetracycline resistance transcriptional repressor (tetR), which is registered by 10_Heidelberg iGEM team (BBa_K337028: tet Repressor).

SsrA is a degradation tag, added at the 3' end of tetR that degrades the repressor protein tetR to become tetR-ssrA, thus we endow the original part a new function as improvement.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 92
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 734
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 92
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 92
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None