Regulatory

Part:BBa_K228004:Design

Designed by: Lin Min   Group: iGEM09_PKU_Beijing   (2009-09-18)
Revision as of 04:18, 13 October 2009 by Spring zhq (Talk | contribs) (→‎Design Notes)

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NahR( reverse) - salicylate promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 408
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is necessary to notice that there is no terminator downstream of the NahR coding sequence, but it has no effect on parts that are in the same direction as the pSal promoter. However, if coding gene is placed upstream of this part and in the same direction as NahR, it may cause unexpected expression.

Source

It is constrcuted by PCR from the plasmid kindly provided by Voigt Lab in UCSF.

References