Regulatory

Part:BBa_K118011

Designed by: Andrew Hall   Group: iGEM08_Edinburgh   (2008-08-19)
Revision as of 07:14, 12 October 2022 by ParadoxDecade (Talk | contribs)


PcstA (glucose-repressible promoter)

This is the promoter for the Eschaerichia coli JM109 cstA gene. It includes the CRP-binding site and the RNA polymerase-binding site. Low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to associate with the promoter and promote transcription of the downstream gene. (cstA encodes the carbon starvation protein.)


Usage and Biology

ShanghaiTech_China 2022's Characterization

domestication.
Figrure 1.The result of sfGFP (with LVA) expression under the conditions of different carbon source concentrations

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//collections/probiotics/control
//direction/forward
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
Parameters
negative_regulators
positive_regulators1