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Part:BBa_K4117666:Experience
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Applications of BBa_K4117666
We added the GFP downstream of the RNA thermometer (RBS) to characterize the efficiency of RNA thermometer.
![T--BNU-China--GFPpathway.png](/wiki/images/b/b4/T--BNU-China--GFPpathway.png)
In order to verify the function of RNA thermometers, We cultured the strain at 37°C overnight, and then aliquots, adding pre-prepared flasks filled with 37°C and 28°C LB medium to make the final volume 20ml, then measured the initial OD600 and GFP fluorescence intensity. Samples were taken once every half-hour, OD600 and GFP fluorescence intensity were measured.
GFP measurement results are shown in Fig.2.
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