Coding

Part:BBa_K4275031

Designed by: Maggie Li   Group: iGEM22_GreatBay_SCIE   (2022-10-10)
Revision as of 07:02, 12 October 2022 by Jerryatcg (Talk | contribs)


PfuFerritin-wt

Ferritins are present in a wide variety of organisms: bacteria, archaea, fungi, plants, insects and vertebrates. Their main role is presumably iron storage; excess of Fe2+ from a cell environment is internalized and then taken up by ferritin, oxidized, and stored as ferrihydride in its cavity until the cell needs it.

Usage and Biology

Ferritin has properties of an longer half life and good thermostability meaning that it can be used for nanobody displays. Most importantly in our project, ferritin can be used to magnetophoresis which is plays the role of recollecting microbials in a reactive solution. Because ferritin mainly exists in the cytoplasm of microbials, it the iron component in the cell functions to locate the bacteria in our project.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None