Composite

Part:BBa_K4229065

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-30)
Revision as of 06:17, 12 October 2022 by NikitaEdel (Talk | contribs)

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mTurquoise2 with C-terminal SnoopCatcher and RBS

mTurqouise2 is with mVenus2(BBa_K4229063) one of our reporter genes, with which we tested the building and catching of the minimal(BBa_K4229047) and full wiffleball(BBa_K4229049) and SPD5 (BBa_K4229078). It has the snoopCatcher fused on the C-terminal of the protein to make it recruitable inside those compartments. For experimental data look into Biobrick BBa_K4229067.


Principle of catching proteins via Spy/Snp-Catcher by T1 and their incorporation into the BMCs, illustrated as an example of incorporating mVenus in the full wiffleball




















Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 783
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 783
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 783
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 783
    Illegal AgeI site found at 48
    Illegal AgeI site found at 944
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None