Coding

Part:BBa_K4275026:Design

Designed by: Yuxuan Bai   Group: iGEM22_GreatBay_SCIE   (2022-09-30)
Revision as of 06:12, 12 October 2022 by Jerryatcg (Talk | contribs)


Neae-Nb3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. The export tag situated on the N terminal of the Neae-Nb3 fusion construct directs the transporation of the target protein to the outer membrane of the bacteria from periplasm.

2. The beta-barrel domain (Figure 1) in the Neae region spans accross the outer memrbane of E.Coli and provides structural support for the whole construct.

3. A spacer domain is situated between the C terminal Nb3 domain and the transmembrane beta-barrel domain.

Source

Escherichia coli
(Neae) Lama glama (Nb3)

References

1. Glass, David S, and Ingmar H Riedel-Kruse. “A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns.” Cell vol. 174,3 (2018): 649-658.e16. doi:10.1016/j.cell.2018.06.041