DNA

Part:BBa_K4438173

Designed by: Diya Raj N   Group: iGEM22_IISER-Tirupati_India   (2022-10-12)
Revision as of 05:54, 12 October 2022 by Registry (Talk | contribs)

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FASTmiR-27a-D1_p

FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. This is the expression cassette for BBa_K4438113 with BBa_J64997 upstream. FASTmiR-27a-D1_p, after transcription, binds to miR-27a-5p, the transcription product of BBa_K4438502. The binding of these to RNAs will lead to the revelation of a binding site for DFHBI, a fluorophore, where it gets trapped and shows fluorescence.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 72
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 72
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 72
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 72
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None