Coding

Part:BBa_K4274010

Designed by: Jiawen Chen   Group: iGEM22_KEYSTONE   (2022-09-30)
Revision as of 05:13, 12 October 2022 by EmmaChen (Talk | contribs)

degQ

degQ gene is pleiotropic regulator gene encoding a 46 amino acid polypeptide in Bacillus subtilis 168. It can regulate the expression of a variety of exocrine enzymes and the production of antibacterial substances. Previous studies have shown that the importance of degQ gene in fengycins production. Therefore, degQ gene was knocked-in Bacillus subtilis 168 together with sfp gene from Bacillus amyloliquefaciens FZB42 (Part: BBa_K4274009).

Usage and Biology

degQ gene (Gene ID: 936547) is pleiotropic regulator gene encoding a 46 amino acid polypeptide in Bacillus subtilis 168. It can regulate the expression of a variety of exocrine enzymes and the production of antibacterial substances. It is reported that increased expression of degQ in B. subtilis 168 results in a 7-10 fold increase in antibiotic production. It is because a T base at position -10 in the promoter region of degQ is mutated into a C, resulting in the inability to express degQ. In this case, DNA sequence of degQ gene under the regulation of p43 promoter was knocked-in the region of the natural sfp gene of B. subtilis. It was used in the composite part PvanP*-sfp_target-sfp_HA_US-p43-K4274013-sfp-K4274014-degQ-sfp_HA_DS (Part: BBa_K4274035) to realize fengycins’ production in B. subtilis. This can be used for other teams working on fengycin production or research on the antibiotic properties of cyclolipopeptides.


Source

B. amyloliquefaciens FZB42

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 38
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 38
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 38
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 38
    Illegal NgoMIV site found at 95
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 661

References

[1]Chen X.H., Koumoutsi A., Scholz R., et al. More than Anticipated – Production of Antibiotics and Other Secondary Metabolites by Bacillus amyloliquefaciens FZB42. Mircrobial Biotech. 16 (2), 14-24 (2009). https://doi.org/10.1159/000142891.

[2]Jin P., Wang H., Liu W., et al. Characteriztion of IpaH2 gene corresponding to lipopeptide synthesis in Bacillus amyloliquefaciens HAB-2. BMC Microbio. 17 (2), 227 (2017). https://doi.org/10.1186/s12866-017-1134-z.

[3]Tsuge K., Ano T., Hirai M., et al. The Genes degQ, pps, and Ipa-8(sfp) Are Responsible for Conversion of Bacillus subtilis 168 to Plipastin Production. Antimicrobial Agents and Chemo. 43(9), 2183-2192 (1999). https://doi.org/10.1128/AAC.43.9.2183.

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