![](https://parts.igem.org/images/partbypart/icon_plasmid_backbone.png)
Plasmid_Backbone
Part:BBa_K4201015
Designed by: David Birkhaeuser, Lindsey Neukirch Group: iGEM22_CU-Boulder (2022-10-11)
Kan BsaI
Our Kan backbone is based on a vector which has previously been shown to be effective in agrobacterium transformation, pLSUK1. The backbone contains several genes from the pSV1 replicon, which is responsible for replication and genetic stability in agrobacterium2. This backbone served as our final vector for a gene of interest to be used in EHA105 transformation competent agrobacterium. The gene contains a blue/white selection marker from pUC193 which is flanked by BsaI cut sites to allow for Golden Gate assembly, as well as a kanamycin resistance gene for antibiotic selection.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 354
Illegal XbaI site found at 327
Illegal PstI site found at 315 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 354
Illegal NheI site found at 804
Illegal PstI site found at 315
Illegal NotI site found at 849
Illegal NotI site found at 2139 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 354
Illegal BamHI site found at 333 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 354
Illegal XbaI site found at 327
Illegal PstI site found at 315 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 354
Illegal XbaI site found at 327
Illegal PstI site found at 315
Illegal NgoMIV site found at 1747
Illegal NgoMIV site found at 1892
Illegal NgoMIV site found at 2103
Illegal NgoMIV site found at 2325
Illegal NgoMIV site found at 2571
Illegal NgoMIV site found at 2660
Illegal NgoMIV site found at 2800
Illegal AgeI site found at 1054 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 750
Illegal BsaI.rc site found at 68
Illegal SapI site found at 922
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Categories
Parameters
None |