Composite

Part:BBa_K4444003

Designed by: June Ahn, Moses Zeidan   Group: iGEM22_UPenn   (2022-10-10)
Revision as of 04:26, 12 October 2022 by Ciperez (Talk | contribs)


PhoCl Shed GFP

PhoCl Shed mRuby is modified from the RELEASE system. It contains a GFP as a protein of interest, PhoCl2c, and a tri-transmembrane domain; the ER retention motif, furin cut site, and HCV cut site were all removed. PhoCl Shed will keep the protein of interest on the cell membrane until violet light is shined on it, which then breaks PhoCl and releases all the protein from the surface of the cell membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 52
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 775
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1660
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 60
    Illegal BsaI.rc site found at 1294


[edit]
Categories
Parameters
None