Coding
NisQ

Part:BBa_K4437004

Designed by: Danielle Korsrud   Group: iGEM22_Calgary   (2022-09-30)
Revision as of 04:21, 12 October 2022 by Danikorsrud771 (Talk | contribs)


Antimicrobial region of nisin Q

This part contains the coding sequence for the antimicrobial region of NisQ, a variant of nisin.

Derived from Lactococcus lactis, nisin is a food-safe, antimicrobial peptide (AMP) that targets a wide range of gram positive bacteria by binding to lipid II on the pathogens membrane, creating a pore, and causing cell death. Literature suggests that nisin Q (NisQ) demonstrates greater antimicrobial and antioxidant activity against pathogens compared to other variants of nisin, such as NisA (BBa_K1365000). Nisin’s optimal pH stability is between 2 and 7 but can maintain its antibacterial activity up to a pH of 12, and can also retain its antimicrobial activity at temperatures of 120oC.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 37
  • 1000
    COMPATIBLE WITH RFC[1000]

This sequence contains only the antimicrobial region of the peptide nisin Q.


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Categories
Parameters
None