Composite

Part:BBa_K4509569:Design

Designed by: MARIE JYOTSNA JAWAHAR, Vibha Murali, Rhea Harry William   Group: iGEM22_REC-CHENNAI   (2022-09-28)
Revision as of 04:15, 12 October 2022 by Marie JJ (Talk | contribs) (→‎Design Notes)


HORSERADISH PEROXIDASE with constitutive promoter J23100


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 445
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 565
    Illegal AgeI site found at 719
    Illegal AgeI site found at 1022
  • 1000
    COMPATIBLE WITH RFC[1000]


Source and Design

Source: BBa_K1291071

The promoter J23119 is replaced with J23100. This switch in the design will reduce the burden in the cell during over expression of enzymes.

Charcaterization

Growth Curve

hrp-100-1.png

References