Part:BBa_K4322002

csgAα-asPink fusion protein with glycine-serine linker

When csgA-alpha fusion chromoproteins are expressed in a culture of E. coli which is then co-cultured with cells secreting csgA-gamma(BBa K4322006), polymers of csgA-alpha-chromoprotein and csgA-gamma will form. The fibrin knob domain/alpha (BBa K4322001) and the fibrin hole domain/gamma (BBa K4322002) will interact (as they do in fibrin [1]) to form long polymers that are covalently linked to chromoproteins [2]. This part is a fusion of the csgA-alpha fusion protein (BBa_K4322005), a glycine-serine linker sequence, and the asPink chromoprotein (BBa_K1033927).

References:

[1]R. I. Litvinov et al., “Polymerization of fibrin: direct observation and quantification of individual B:b knob-hole interactions,” Blood, vol. 109, no. 1, pp. 130–138, Jan. 2007, doi: 10.1182/blood-2006-07-033910.

[2]A. M. Duraj-Thatte et al., “Programmable microbial ink for 3D printing of living materials produced from genetically engineered protein nanofibers,” Nat Commun, vol. 12, no. 1, p. 6600, Dec. 2021, doi: 10.1038/s41467-021-26791-x.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 1388
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 1388
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 1388
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 1388
  Illegal NgoMIV site found at 58
  Illegal NgoMIV site found at 1193
  
• 1000
  COMPATIBLE WITH RFC[1000]