Composite

Part:BBa_K4472988

Designed by: Stine Behrmann   Group: iGEM22_Uni-Hamburg   (2022-10-11)
Revision as of 03:14, 12 October 2022 by Registry (Talk | contribs)

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Split ribozyme detecting hcat and expressing eforRED

This composite part is build out of the split ribozyme halfs, guide RNAs complementary to the stable endogenous E.coli mRNA hcat and an eforRED reporter. We assembled this part using golden gate assembly and transformed it into E.coli DH5alpha.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 331
    Illegal NheI site found at 354
    Illegal NheI site found at 686
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 588
    Illegal XhoI site found at 135
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None