Composite

Part:BBa_K4390105

Designed by: Maarten van den Ancker   Group: iGEM22_Edinburgh-UHAS_Ghana   (2022-09-21)


arsR controlled Broccoli biosensor

Improvement of part BBa_K3380600

This part is an improvement of Part:BBa_K3380600, as it uses a brigther aptamer. We designed a TXO ArsR-regulated T7 promoter biosensor expressing Broccoli instead of iSpinach (Part:BBa_K4390105). Broccoli binds DFHBI more tightly than iSpinach, hence producing brighter fluorescence (See Figure 1).


Various aptamers.png
Figure 1: The brightness of various aptamers in combination with various fluorophores. The column labelled "Green" represents iSpinach.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 52
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 52
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 52
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 52
  • 1000
    COMPATIBLE WITH RFC[1000]


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