Device

Part:BBa_K4472991

Designed by: Stine Behrmann   Group: iGEM22_Uni-Hamburg   (2022-10-11)
Revision as of 02:50, 12 October 2022 by Registry (Talk | contribs)

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Split ribozyme detecting hcat and expressing GFP

This composite part is build out of the split ribozyme halfs, guide RNAs complementary to the stable endogenous E.coli mRNA hcat and a GFP reporter. We assembled this part using golden gate assembly and transformed it into E.coli DH5alpha.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 331
    Illegal NheI site found at 354
    Illegal NheI site found at 686
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 588
    Illegal XhoI site found at 135
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1495


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Parameters
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