Part:BBa_K1847001:Experience

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Characterization and Improvement by LZU-CHINA iGEM 2022

First, we successfully isolated this part and verified it by gel electrophoresis.

Figure 1: Agarose gel electrophoresis result of lldr.

Then,we built on this part by introducing a combination of hyper-spank and RBS spoVG in its head and an optimized sequence of RiboJ in its tail, once created a new LlprD operon( BBa_K4156101 ). Most importantly, we combined the constructed LlprD operon with the TP901-mediated XOR logic gate, which greatly improved the performance of the LlprD operon.

The optimized LlprD operon improves the signal output level while the signal output level is more stable. This is undoubtedly beneficial for the subsequent construction. More notably, we introduced the TP901-XOR gate system, which helps to coordinate many different inducible operons.We characterized our optimized system using LlprD-mRFP as a control, characterization details can be found at

pLldR Operon BBa_K4156101  ;

pLldR-mRFP BBa_K4156114  ;

pLldR-TP901 BBa_K4156104  ;

pLldR-TP901-φ174E BBa_K4156105

and XOR-gate BBa_K4156117

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