Device

Part:BBa_K4472993:Design

Designed by: Stine Behrmann   Group: iGEM22_Uni-Hamburg   (2022-10-11)
Revision as of 02:39, 12 October 2022 by Registry (Talk | contribs)

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guideRNA 2 detecting hcat


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The guideRNA should have a length of 82 basepairs (Gambill L, Staubus A, Ameruoso A, Chappell J. A split ribozyme that links detection of a native RNA to orthogonal protein outputs. bioRxiv. January 2022:2022.01.12.476080. doi:10.1101/2022.01.12.476080)


Source

The guide RNA is complementary to the stable endogenous E.coli mRNA hcat.

References