Generator

Part:BBa_K4241804

Designed by: Vicera Christian Nelson Tejedor   Group: iGEM22_HKU_HongKong   (2022-10-11)
Revision as of 02:20, 12 October 2022 by Registry (Talk | contribs)

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CvNDI1_Protein generator (microalgae)

This part is CvNDI1, a nitrogen starvation induced promoter from Chlorella vulgaris to be used in microalgal expression chassis. This combines the UTR and terminator for a combined protein generator. This part is derived from the following publication: https://www.nature.com/articles/s41598-020-69620-9

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1366
    Illegal PstI site found at 55
    Illegal PstI site found at 404
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1366
    Illegal PstI site found at 55
    Illegal PstI site found at 404
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1366
    Illegal PstI site found at 55
    Illegal PstI site found at 404
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1366
    Illegal PstI site found at 55
    Illegal PstI site found at 404
    Illegal NgoMIV site found at 264
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 849


[edit]
Categories
Parameters
None