Composite

Part:BBa_K4241802

Designed by: Vicera Christian Nelson Tejedor   Group: iGEM22_HKU_HongKong   (2022-10-11)
Revision as of 02:11, 12 October 2022 by Registry (Talk | contribs)

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CvNDI1_CeB_TRAmy3D

This part is used for the inducible expression of Cecropin B in a high-expression manner in microalgae. CvNDI1 is a strong promoter induced via nitrogen starvation. This promoter is derived from the urea carboxylase, and can be used to increase expression up to 20-fold as compared to wild-type expression.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1506
    Illegal PstI site found at 55
    Illegal PstI site found at 404
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1506
    Illegal PstI site found at 55
    Illegal PstI site found at 404
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1506
    Illegal PstI site found at 55
    Illegal PstI site found at 404
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1506
    Illegal PstI site found at 55
    Illegal PstI site found at 404
    Illegal NgoMIV site found at 264
    Illegal NgoMIV site found at 1086
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 849


[edit]
Categories
Parameters
None