Part:BBa_K4438701
P4G13_trigger_1_w/oPol
P4G13_trigger_1_w/oPol is single-stranded DNA having 38 nucleotides. Figure 1B) shows the secondary structure and minimum free energy. At the 5’ end, a few bases are complementary to P4G13_aptamer (BBa_K4438700). The middle region consists of a full ssDNA sense T7 promoter sequence and at the 3’ end, there are a few bases of ssDNA sense broccoli sequence.
Usage and Biology
Figure 2D) Shows the secondary structure of both the parts hybridised at 37° Celsius. The hormone binds with P4G13_aptamer (BBa_K4438700) with high affinity [1] and displaces the P4G13_trigger_1_w/oPol (BBa_K4438701). This part has complete complementarity with the part P4G13_Target_1 (BBa_K4438702). T7 polymerase binds and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers. All three parts can be used to detect different concentrations of progesterone.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Contreras Jiménez, G., Eissa, S., Ng, A., Alhadrami, H., Zourob, M., & Siaj, M. (2015). Aptamer-based label-free impedimetric biosensor for detection of progesterone. Analytical chemistry, 87(2), 1075-1082
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