Composite
Part:BBa_K4217003
Designed by: Madeline Gruenberg Group: iGEM22_WrightState (2022-09-26)
Revision as of 01:28, 12 October 2022 by Wright-mpc (Talk | contribs)
mntP-riboswitch-sfGFP-double terminator
General Description:
This composite part is a functional biosensor for manganese in water samples. The part consists of the mntP manganese-responsive promoter, riboswitch and ribosome binding site from E.coli, driving expression of sfGFP. The biosensor was inserted into pSB3K3 by the 2022 Wright State iGEM team and shown to detect manganese chloride down to 0.01mM (500ppm). The sensor performance was enhanced with the co-expression of the pTrc-6X-HIS-mntR manganese regulatory protein part BBa_K4217005.
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