![](https://parts.igem.org/images/partbypart/icon_reporter.png)
Part:BBa_K4201012:Design
RUBY
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3745
Illegal BamHI site found at 2394 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 330
Illegal NgoMIV site found at 909
Illegal NgoMIV site found at 2622 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 3968
Design Notes
![](/wiki/images/d/d3/RUBY_pathway.png)
Codon optimization for Glycine max (soybean) is a unique design consideration for all of CU Boulder’s coding sequences including our RUBY. Codon optimization is the intentional use of specific codons for specific amino acids, dependent on what tRNAs are most abundant in the organism. While codon optimization is a common consideration for synthetic biologists, our sequences are unique for iGEM because they are intended for expression in soybeans.
Source
The RUBY sequence contains CYP76AD1, DODA, and Glucosyltransferase coding sequences1. All of our DNA fragments are obtained via de novo synthesis by iGEM sponsors Twist Bioscience and Integrated DNA technologies.
References
1. He, Y., Zhang, T., Sun, H., Zhan, H. & Zhao, Y. A reporter for noninvasively monitoring gene expression and plant transformation. Hortic. Res. 7, 152 (2020).
2. Gandía-Herrero, F. & García-Carmona, F. Biosynthesis of betalains: yellow and violet plant pigments. Trends Plant Sci. 18, 334–343 (2013).