Coding

Part:BBa_K4060021

Designed by: Yan-Zhen, Chen   Group: iGEM21_NYCU-Taipei   (2021-09-30)
Revision as of 01:10, 12 October 2022 by XueZhang (Talk | contribs)


pelB leader sequence with aprN (without native signal peptide sequence)

pelB leader sequence with aprN used signal peptide gene of PelB Helps nattokinase secretion. The propeptide has been proved to function as an intramolecular chaperone and is essential for guiding the correct folding of the linked protein to its mature form.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 67
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 67
    Illegal NotI site found at 211
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 67
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 67
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 345
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 349


[edit]
Categories
Parameters
None