Coding

Part:BBa_K4139002:Design

Designed by: Emily Hagens   Group: iGEM22_Lethbridge   (2022-10-11)
Revision as of 01:01, 12 October 2022 by Registry (Talk | contribs)

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mcyH crRNA for Lbu Cas13a (for E.coli)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 42
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to determine which region of the mcyH gene our crRNA should target, we used the RNAfold tool. This allowed us to locate a target sequence that met the ideal design criteria of being close to the 5' end of the sequence, being relatively unfolded region in the mRNA secondary structure, and having a high probability of remaining single-stranded. This crRNA coding region was designed to be the reverse compliment of the region it will target.


Source

This part was designed using a segment of the mcyH gene as a reference (GenBank: AAF00956.1)

References