Coding
Part:BBa_K4060021
Designed by: Yan-Zhen, Chen Group: iGEM21_NYCU-Taipei (2021-09-30)
pelB leader sequence with aprN (without native signal peptide sequence)
The expression of Nattokinase in Escherichia coli is prone to the formation of inclusion bodies, so we use weak promoter and RBS combination to express Nattokinase. In order to have enough time and space to fold and secrete nattokinase into the intestine.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 67
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 67
Illegal NotI site found at 211 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 67
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 67
Illegal NgoMIV site found at 54
Illegal NgoMIV site found at 345 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 349
[edit]
Categories
Parameters
None |