Part:BBa_K4169015

produce TMADH

After expressing, it'll produce trimethylamine dehydrogenase (TMADH (EC 1.5.99.7)). The enzyme TMADH is an iron–sulfur flavoprotein which catalyses the oxidative demethylation of trimethylamine (TMA) to dimethylamine and formaldehyde:
(CH₃)₃N + H₂O → (CH₃)₂NH + CH₂O +2H⁺ + 2e^-.

Metabolic Pathway

Figure 1.Pathways for trimethylamine metabolism in bacteria.

Figure 2.Protein molecular structures of trimethylamine dehydrogenase.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 388
  Illegal PstI site found at 183
  Illegal PstI site found at 1782
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 388
  Illegal PstI site found at 183
  Illegal PstI site found at 1782
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 388
  Illegal XhoI site found at 1717
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 388
  Illegal PstI site found at 183
  Illegal PstI site found at 1782
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 388
  Illegal PstI site found at 183
  Illegal PstI site found at 1782
  Illegal AgeI site found at 879
  
• 1000
  COMPATIBLE WITH RFC[1000]