Coding
T5OH
Part:BBa_K4201007
Designed by: Maya Li Nelson Group: iGEM22_CU-Boulder (2022-10-01)
T5αOH
The Taxane 5α-hydroxylase (T5αOH) naturally occuring in Yew trees metabolizes Taxadiene, the precursor molecule to Paclitaxel, into Taxadiene-5α-ol1. T5αOH is the second step in the synthesis pathway of paclitaxel1. Successful integration of this enzyme could help further the progress of the biosynthesis of paclitaxel.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 184
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 184
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 184
Illegal BglII site found at 800 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 184
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 184
Illegal NgoMIV site found at 333 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 1517
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Categories
Parameters
None |