Composite

Part:BBa_K4235030

Designed by: Maulik Masaliya, Lori Saxena, Stephanie Laderwager   Group: iGEM22_Stony_Brook   (2022-09-21)
Revision as of 23:18, 11 October 2022 by Maulik masaliya (Talk | contribs) (→‎Characterization and results)

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PROS1 expression cassette

This is the entire expression circuit for the Human protein S gene.

Ecoli circuit.png

Characterization and results

We successfully cloned our insert BBa_K4235011 into the pET His6 vector BBa_K4235016 using Ligation independent cloning. Please refer the PROS1 insert page BBa_K4235011 for results.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1989
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1159
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/biosynthesis
//chassis/prokaryote
//function/biosynthesis
Parameters
chassisEscherichia coli