DNA
Part:BBa_K4274007
Designed by: Jiawen Chen Group: iGEM22_KEYSTONE (2022-09-30)
CmR29M2-ClSS_S533A
ClSS is a biobrick part encoding the gene for alpha-santalene synthase from Clausena lansium (GenBank: ADR71055.1). The enzyme catalyze the conversion of the common isoprenoid intermediate farnesyl pyrophosphate (FPP) into the alpha-santelene in a single step.The gene has been demonstrated to produce functional terpene product in both yeast (Wenlong Z., 2020) and E. coli (Jia Z. et al., 2022) .
CmR29, a DNA fragment comprising 87 nucleotides from the 5′end of the CmR gene, is a competent solubility enhancer in Synechocystis (Nico Betterle et al., 2018). CmR29M2 is a mutant CmR29 polypeptide for the expression of 3 different amino acids(Xun W. et al.,2021). Due to more than half of heterologous protein aggregated into insoluble inclusion bodies is an important limiting factor in the E. coli expression system, CmR29M2 fusion tag was linked to the N-terminus of ClSS, and the production of α-santalene reached 131% of the no-tag strain (Jia Z. et al., 2022).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 270
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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