Coding

Part:BBa_K4165256

Designed by: Esraa Elmligy   Group: iGEM22_CU_Egypt   (2022-10-08)
Revision as of 22:17, 11 October 2022 by SalmaMohsen 2000 (Talk | contribs)


GST-Coh2-G4S-WWW

Long description

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85

WetLab Results

Transformation of GST COH WWW in BL-21 using pGS-21a vector 

                                    Figure 1. Transformed plate of GST COH (L) WWW + pGS-21a

Transformation of GST COH WWW in DH-5 alpha using pJET vector

                                   Figure 2. Transformed plate of GST COH (L) WWW + pJET

Comparison between chemical lysis and sonication for GST COH WWW

Figure 3. This graph shows a significant difference between chemical lysis and sonication for GST COH WWW, after we had the results we optimized our protocol to use chemical lysis for GST COH WWW

SDS PAGE for induced and non induced samples of GST COH WWW

Figure 4. This figure shows the comparison between induced and non induced samples of GST COH WWW, where well no.1 is the induced sample while well no.5 is the non induced sample showing that our protein is induced effectively owing to our right choice of IPTC, time interval and concentration


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