Reporter

Part:BBa_K734002:Experience

Designed by: Logan Bachman   Group: iGEM12_Austin_Texas   (2012-08-22)
Revision as of 22:13, 11 October 2022 by Natalietan (Talk | contribs) (→‎Applications of BBa_K734002)


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Applications of BBa_K734002

Lambert_GA 2022

Lambert iGEM attempted to characterize Spinach to validate the improvements of iSpinach-D5-G30-A32 No part name specified with partinfo tag. and iSpinach No part name specified with partinfo tag. . iSpinach is a mutant of Spinach developed by researchers at the University of Strasbourg. Compared to Spinach, iSpinach is less salt-sensitive, has higher thermal stability, and produces more fluorescence (Autour et. al, 2016). iSpinach-D5-G30-A32 is a co-crystallized, re-engineered version of iSpinach developed by researchers at the University of Strasbourg. We created No part name specified with partinfo tag., a composite part producing Spinach; No part name specified with partinfo tag. , a composite part producing iSpinach; and No part name specified with partinfo tag. , a composite part producing iSpinach-D5-G30-A32. The only difference is the basic aptamer part, allowing us to accurately compare the aptamers. However, after failed attempts at cloning, Lambert iGEM decided to continue the characterization of the various Spinach aptamers in 2023.

Reference

Autour, A., Westhof, E., & Ryckelynck, M. (2016). iSpinach: A fluorogenic RNA aptamer optimized for in vitro applications. Nucleic Acids Research, 44(6), 2491–2500. https://doi.org/10.1093/nar/gkw083

User Reviews

UNIQ5f2a19730b180ec3-partinfo-00000005-QINU UNIQ5f2a19730b180ec3-partinfo-00000006-QINU