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Coding
Part:BBa_K4165255
Designed by: Esraa Elmligy Group: iGEM22_CU_Egypt (2022-10-08)
Revision as of 22:12, 11 October 2022 by SalmaMohsen 2000 (Talk | contribs)
GST-Coh2-G4S- TD28REV
Long description
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 689
Illegal SapI.rc site found at 85
WetLab Results
Transformation of GST COH (L) TD28 Rev in BL-21 using pGS-21a vector
Figure 1. Transformed plate of GST COH (L) TD28 Rev + pGS-21a
Transformation of GST COH (L) TD21 Rev in DH-5 alpha using pJET vector
Figure 2. Transformed plate of GST COH (L) TD28 Rev + pJET
Comparison between chemical lysis and sonication for GST COH TD28 Rev
SDS PAGE of induced and non induced samples of GST COH TD28 Rev
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Categories
Parameters
None |