Coding
Part:BBa_K4229001
Designed by: Nikita Edel Group: iGEM22_Freiburg (2022-09-28)
Fre (NAD(P)H-flavin reductase)
The flavin-reducing enzyme Fre catalyzes the reduction of soluble flavins by reduced pyridine nucleotides (UniProt ID:P0AEN1).
Fre is one of four enzymes we used in our project to produce indigo and indirubin. In our pathway it accelerates the recycling of the cofactor NADPH, and adds more FADH2 for the enzyme XiaI.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 304
Illegal SapI.rc site found at 424
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Categories
Parameters
None |