Composite

Part:BBa_K4245202

Designed by: Akshaya Poonepalle, Shivaek Venkateswaran, Varnica Basavaraj, Manaswi Gorle, Sahana Ram Narayanan, Janet Standeven   Group: iGEM22_Lambert_GA   (2022-10-09)
Revision as of 21:19, 11 October 2022 by RichardJiang (Talk | contribs)

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hsa-miR-122-5p RCA Padlock Probe

This part is the sequence for the hsa-miR-122-5p RCA Padlock Probe, which our team designed to be specific to BBa_K3876501, hsa-miR-122-5p. This miRNA acts as a biomarker for coronary artery disease, and is therefore potentially useful for the early detection of this condition. When using the Rolling Circle Amplification approach, the produced transcript contains repeats of the middle sequence (to which the DNA fluorescent aptamer split lettuce (BBa_K4245134/ BBa_K4245135) and the FAM and BHQ1 labeled linear probes (BBa_K4245130/BBa_K4245132) bind) and the 3’ (BBa_K4245101) and 5’(BBa_K4245108) arms of the padlock probe.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None