Regulatory

Part:BBa_K180000

Designed by: Richard Wilson, Stephanie Chambers   Group: iGEM09_Southampton   (2009-10-12)
Revision as of 14:06, 12 October 2009 by Registry (Talk | contribs)

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Hybrid promoter (trp & lac regulated -- tac pR)

The Ptac promoter is a functional hybrid promoter, derived from the trp and lac promoters, that are regulated by trp and lac [1]. The Ptac sequence has been extracted by PCR from the commercially available plasmid pMAL-pIII from New England Biolabs (N8101S), with the addition of the biobrick prefix and suffix sequences.

[1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
Parameters
controltrp, lac
directionforward
n/aHybrid promoter (trp & lac regulated -- tac pR)