Composite

Part:BBa_K200009:Design

Designed by: David Roche, Royah Vaezi   Group: iGEM09_Imperial College London   (2009-08-12)
Revision as of 08:12, 12 October 2009 by Royah (Talk | contribs) (→‎Source)

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DpnII Composite


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NOTE: Unlike other restriction enzymes that show Star Activity at high pH 8.0, DpnII has Star Activity above approximately pH 6.5. The effect of pH on Star Activity of DpnII is very extreme, showing one-thousand-fold more Star Activity at pH 7.5 than at pH 6.0.

Source

Streptococcus pneumoniae Hungary19A-6.

The DNA sequence was then synthesised and includes a RBS preceding the coding region for DpnII.

References

http://www.ncbi.nlm.nih.gov/nuccore/NC_010380.1?report=fasta&from=1825422&to=1826288