Composite

Part:BBa_K4388005:Experience

Designed by: Tomas Lopes   Group: iGEM22_KCL_UK   (2022-10-09)
Revision as of 19:12, 11 October 2022 by KalyanGhadiyaram (Talk | contribs) (→‎Applications of BBa_K4388005)


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Applications of BBa_K4388005

KCL iGEM 2022

Through JUMP type IIS Level 1, this transcriptional unit (BBa_K4388005) was assembled in a one-pot digestion reaction with BsaI and ligation into pJUMP29-1A. Successful ligation and transformation were selected for by identifying non-fluorescent colonies on kanamycin agar plates (Fig. 1).

"A E.coli high quality.png Figure 1. Construction of Transcriptional Unit (TU) BBa_K4388005 into Level 1 pJUMP29-1A vector. Level 0 parts BBa_K4388000, BBa_K4388001, and BBa_K4388012 were assembled into the transcriptional unit BBa_K4388005 in pJUMP29-1A via a one-pot BsaI digestion reaction. White (sfGPF-excised) colonies indicate successful ligation of the TU into plasmid, and successful transformation of plasmid conferring kanamycin resistance. Final level 1 plasmid constructs were designed using SnapGene.

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